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KMID : 1024420150190020111
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Food Engineering Progress
2015 Volume.19 No. 2 p.111 ~ p.116
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Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
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Cho A-Young
Kim Kee-Young
Lim Jong-Guk
Mo Chang-Yeun
Moon Ji-Hye
Park Saet-Byul
Park Su-Hee
Om Ae-Son
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Abstract
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The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work-Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies.
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KEYWORD
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staphylococcal enterotoxin B (SEB) , fast protein liquid chromatography (FPLC) , separation , purification , detection
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